6.__Endocrinology - Peer Pods: Endocrinology ACTIVITY 1:...

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Endocrinology ACTIVITY 1: WHATS HAPPENING IN THIS PICTURE AND THAT SCENARIO (10 MIN.) Work as a group to answer the following questions. Remember, it is important to get all members of your pod to participate. You can either assign each point to a different person or pair, or you can work on them as a larger group, but you must demonstrate to your TA that everyone in your pod is able to answer all questions. A. As we did last week, tell the story of the G-Protein Coupled Receptor by describing what is happening in the picture your TA will pass around. Be sure to identify all the components (including what is covered up). B. Predict what would happen to the process if the molecule labeled “A” were to mutate so that it could no longer bind with the molecule labeled “B.” C. Epinephrine uses the GPCR to stimulate a response in many different types of tissue including liver, muscle, and heart. In all these tissues, epinephrine binds to identical receptors and the receptors elicit the same second messenger response, but produces different effects. How can epinephrine use the same signaling pathway but cause a different effect in each tissue. TA Key: This activity should bring back memories of recitation. The students need to practice articulating/describing multi-step processes. A. The figure shows the G-Protein Coupled Receptor activation pathway with the names of some of the molecules covered up. As you were once asked to do in recitation, the students should be able to clearly describe this process, including naming the structures. Their story should note the following: - Receptor binds to a ligand outside the cell (a highly specific event). - GTP replaces GDP on G Protein; binding of GTP activates G Protein - G Protein splits: beta and gamma subunits come off, alpha/GTP subunit moves through membrane to bind with effector Adenylyl cyclase - Adenylyl cyclase (effector) uses ATP to make cAMP - cAMP = second messenger, activates another protein (PKA) that subsequently acts on a response protein to elicit a cellular response. B. The molecule labeled A is PKA. If it were to mutate or be modified so that it could no longer bind B (which is cAMP), the second messenger would have no effect and the cellular response would not be initiated even though the G Protein is functioning. C. The response protein is a generic term for a number of proteins that would be activated by the second messenger (cAMP). Therefore, different cell types can use a single type of receptor but respond in separate ways. The common feature is that all the different cell types use the specific receptor of the GPCR to bind to a single type of ligand, and cAMP acts as a second messenger for to activate the effector protein (PKA) but the response protein that PKA will activate is varied. ACTIVITY 2:
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6.__Endocrinology - Peer Pods: Endocrinology ACTIVITY 1:...

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