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Unformatted text preview: Biology 1510 Lab Quiz 8 Your Name :___________________________ T.A. Name :____________________________ Lab section, date, time :___________________ This quiz should take no more than 15 minutes. Clearly mark your answers on this sheet. Ambiguous and vague answers will not be counted as correct. 1) 1 point. Many biotechnology companies work with DNA plasmids with the goal of producing large amounts of protein like insulin. Once the gene encoding for the protein insulin is inserted into the plasmid what must be done to get the protein expressed? a. Sequence the gene of interest b. Amplify the gene sequence using PCR c. Input the sequence into BLASTn d. Put the plasmid into bacterial cells ANSWER: D 2) 1 point. One strand of a DNA molecule has the following sequence: 3'--- A G T A C A A A C T A T C C A C C G T C ---5' If this strand were transcribed, the resulting molecule would have the sequence: a. 5'- U C A U G U U U G A U A G G U G G C A G ---3' b. 5'- A G T A C A A A C T A T C C A C C G T C ---3' c. 5'- T C A T G T T T G A T A G G T C C D A ---3' d. 3'- U G A U A U U U G A U A G G U G G C A G ---5' ANSWER: A. The sequence must go anti-parallel, 5 to 3. Remember that mRNA have uracil instead of thymine. Alternate #2 2) 1 point: Indicate the start codon for translation from the mRNA sequence below: 5---U U A G G A A C G A G U A A A C G A U G G C---3 ANSWER: 5---U U A G G A A C G A G U A A A C G A U G G C---3 Initiation codon 3) 2 points. Besides the E-value, name two other important things to consider when trying to determine the identity of your unknown sequence from BLAST results....
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This note was uploaded on 04/29/2008 for the course BIO 1510 taught by Professor Unknown during the Fall '07 term at Georgia Institute of Technology.
- Fall '07