{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Problem Set11 Answers - Genetics 380 Spring 2008 Problem...

Info icon This preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Genetics 380 : Spring 2008 Problem Set 11 Answers 1. The mutant gene should bind more of the transcriptional activator protein or bind it more efficiently. Therefore, the mutant gene should be induced with lower levels of the transcriptional activator protein or expressed at higher levels (make more of the gene product) than the wild-type gene, or both. 2. An inversion not only inverts the sequence, but because the DNA strands in a duplex are antiparallel, also exchanges the strands. The inversion of a promoter sequence would have a profound effect because the mutant promoter is in the wrong strand transcribing in the wrong direction. One would expect little or no effect of the inversion of an enhancer element because enhancers are targets of DNA-binding proteins irrespective of orientation in the DNA. 3. (a) Genotype Phenotype for Gal1p -Galactose +Galactose GAL80 GAL4 GAL1 - + gal80- GAL4 GAL1 + + GAL80 gal4- GAL1 - - GAL80 GAL4 gal1- - - gal80 i GAL4 GAL1 - - GAL80 gal4 r GAL1 + + GAL80 gal4 a GAL1 - - GAL80 gal4 d GAL1 - - gal80- gal4- GAL1 - - gal80- GAL4 gal1- - - GAL80 gal4 r gal1- - - gal80 i gal4 r GAL1 + + gal80- gal4 a GAL1 - - gal80- gal4 d GAL1 - -
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Explanations for the table above- GAL80 gene codes for a repressor (inhibitor), that binds to the Gal4 activator protein and inactivates it.
Image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern