Problem Set11 Answers

Problem Set11 Answers - Genetics 380 Spring 2008 Problem...

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Genetics 380 : Spring 2008 Problem Set 11 Answers 1. The mutant gene should bind more of the transcriptional activator protein or bind it more efficiently. Therefore, the mutant gene should be induced with lower levels of the transcriptional activator protein or expressed at higher levels (make more of the gene product) than the wild-type gene, or both. 2. An inversion not only inverts the sequence, but because the DNA strands in a duplex are antiparallel, also exchanges the strands. The inversion of a promoter sequence would have a profound effect because the mutant promoter is in the wrong strand transcribing in the wrong direction. One would expect little or no effect of the inversion of an enhancer element because enhancers are targets of DNA-binding proteins irrespective of orientation in the DNA. 3. (a) Genotype Phenotype for Gal1p -Galactose +Galactose GAL80 GAL4 GAL1 - + gal80- GAL4 GAL1 + + GAL80 gal4- GAL1 - - GAL80 GAL4 gal1- - - gal80 i GAL4 GAL1 - - GAL80 gal4 r GAL1 + + GAL80 gal4 a GAL1 - - GAL80 gal4 d GAL1 - - gal80- gal4- GAL1 - - gal80- GAL4 gal1- - - GAL80 gal4 r gal1- - - gal80 i gal4 r GAL1 + + gal80- gal4 a GAL1 - - gal80- gal4 d GAL1 - -
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Explanations for the table above- GAL80 gene codes for a repressor (inhibitor), that binds to the Gal4 activator protein and inactivates it. The inducer (a galactose-metabolite) binds to Gal80p and alters it such that it can no longer block Gal4p
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Problem Set11 Answers - Genetics 380 Spring 2008 Problem...

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