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Unformatted text preview: genetic landmarks 1) Genome sequencegenes in this block of DNA in model organisms 2) Chromosome walkfor some species to find and order the clones falling b/t genetic landmarks a) Use sequence of nearby landmark as probe to ID second set of clones that overlaps marker clone containing landmark but extends into two directions b) Series of steps from one adjacent clone to next IV) Polymerase chain reactionto amplify DNA in vitro A) If we know sequence of at least some parts of gene B) Uses multiple copies of primers (15-20 bases long) that bind to different end of gene to be amplified C) Primers bind to opposite DNA strands with their 3 ends pointing at each other, location of primers determines specificity of DNA segment that is amplified D) Taq Polymerase adds bases to primers E) Polymerization process shuttles back and forth b/t them F) Forms exponentially long # of double-stranded DNA molecules...
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This note was uploaded on 04/30/2008 for the course ZOL 341 taught by Professor Dworkin during the Spring '08 term at Michigan State University.
- Spring '08