Practice Midterm I B Key

Practice Midterm I B Key - Name: ID# Midterm I MCDB 165A...

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Unformatted text preview: Name: ID# Midterm I MCDB 165A FALL 2006 BIOLOGY OF THE CELL Name: ID#: 1 TRUE OR FALSE (10 points) 1. MAGUKs comprise a family of tyrosine kinases involved in cell surface signaling events. False 2. Stimulation of fat cells by insulin leads to the endocytosis of glucose transporters. False 3. In eukaryotic organisms the plasma membrane is 5-10 um thick. False 4. The inhibitory G protein (Gi) inhibits adenylyl cyclase by regulating ion channels. True 5. The positive charge of proteoglycans is due to the presence of glycosaminoglycan chains and allows for binding to growth factors. True 6. The structure of both Cadherins and Integrins is calcium-dependent. True 7. Integrins can bind to members of the immunoglobulin superfamily, cadherins and extracellular matrix proteins. False 8. Selectins are required for recruitment of leukocytes to sites of infection. True 9. Type II collagen is present in all cartilages. True 10. Fibronectin is a homotrimeric glycoprotein present in the extracellular matrix. Name: ID#: 1 TRUE OR FALSE (10 points) 1. MAGUKs comprise a family of tyrosine kinases involved in cell surface signaling events. False 2. Stimulation of fat cells by insulin leads to the endocytosis of glucose transporters. False 3. In eukaryotic organisms the plasma membrane is 5-10 um thick. False 4. The inhibitory G protein (Gi) inhibits adenylyl cyclase by regulating ion channels. True 5. The positive charge of proteoglycans is due to the presence of glycosaminoglycan chains and allows for binding to growth factors. True 6. The structure of both Cadherins and Integrins is calcium-dependent. True 7. Integrins can bind to members of the immunoglobulin superfamily, cadherins and extracellular matrix proteins. False 8. Selectins are required for recruitment of leukocytes to sites of infection. True 9. Type II collagen is present in all cartilages. True 10. Fibronectin is a homotrimeric glycoprotein present in the extracellular matrix. False Name: ID#: 2 MULTIPLE CHOICE (30 points) 11. FRET (Fluorescence Resonance Energy Transfer) can be used to study: a) protein-protein interactions b) protein-DNA interactions c) protein conformational changes d) a and b e) all of the above e) 12. Requirements for culture of mammalian cells include all of the following except: a) 5% CO 2 b) adhesive substrate c) adequate nutrients d) incubation temperature 37 C incubation e) mycoplasms e) 13. To observe tissue under a bright field light microscope the specimen must be: a) fixed with paraformaldehyde or other fixatives b) must be stained with Hematoxylin and Eosin (H&E) or other special stains to allow visualization c) must be 5-20 nm thin to allow passage of light d) a, b, e) all of the above d) 14. Cholesterol a) is present in mammalian cells in a 1:1 ratio with phosphoglycerides b) increases fluidity and stability in membranes c) is found in caveolae d) b and c e) all of the above e) 15. Confocal microscopy a) utilizes lasers as a light source b) visualizes specimens thicker than a light microscope...
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Practice Midterm I B Key - Name: ID# Midterm I MCDB 165A...

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