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10-Gene Regulation II

10-Gene Regulation II - Gene Regulation II Eukaryotes pp...

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Gene Regulation II Eukaryotes pp. 454 - 464 Some general thoughts 0. many levels of control exist 1. DNA binding proteins are very important 0. cis vs. trans acting Some general thoughts 2. Eukaryotes do NOT have operons* 3. Both use repressors and activators, but activators VERY common in eukaryotes 4. Chromatin is more unique and therefore plays a role in control Chromatin Structure and Regulation
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DNase I hypersensitivity 5. Brought about by chromatin remodeling 6. No chemical modification 7. Proteins form "chromatin remodeling complex" 8. Binds to DNA and repositions the nucleosomes 9. Exposes different promoters to allow for transcription 10.DNase I digests DNA when it is not tightly bound to histones 11.Transcribed DNA contains "DNase I hypersensitive sites" 1. 1000 nucleotides upstream of transcription start site 12.NOTE: this is an experimental (read: “does not happen in the cell” phenomenon) Histone Acetylation o structure of histones 2 domains 0. globular domain that associates with other histones 1. positively charges tail domain that interacts with phosphates of DNA backbone o addition of acetyl group to histone CH 3 CO- to positive tail domain weakens interaction between DNA and histones added by acetyl transferases removed by deacetylases o
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