Gene Regulation in Prokaryotes

Gene Regulation in Prokaryotes - Gene Regulation in...

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Gene Regulation in Prokaryotes pp. 433 - 453 Gene Regulation Some General Comments o Levels of control DNA RNA pre-transcription processing post-transcriptional o RNA stability Protein translation post-translational modification • see figure 16.1, page 435 Cis vs. Trans - Again! 0. Cis -acting - any sequence of DNA that is not converted into other form but functions as it is in situ , affecting only the DNA to which it is physically linked 1. Trans -acting - the product of a DNA which may be a protein, an RNA. The critical thing - the product (whatever it is) diffuses away
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from its site of synthesis to work elsewhere. Some important lingo o Structural genes o Regulatory genes o Regulatory elements NOT transcribed but still affect gene expression DNA binding proteins o Regulatory proteins have domains discrete functional parts part of the 3-D structure that binds to DNA grouped according to a characteristic structure known as a motif • see figure 16.2, p. 436 Prokaryotic regulation o The operon "a unit of DNA composed of specific genes, plus a promoter and/or operator, that acts in unison to regulate the response of the structural genes to environmental changes" see figure 16.3, p. 437
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Gene Regulation in Prokaryotes - Gene Regulation in...

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