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Pract Prob Set 3B ANSWERS

Pract Prob Set 3B ANSWERS - Practice Problem Set 3B ANSWERS...

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Practice Problem Set 3B ANSWERS Lect 23-26 1. Campbell—Answers in book 2. Some pesticides are highly concentrated in oxidizing compounds. If ingested, these oxidants have the potential to cause _____ mutations ____in human cells by attacking DNA. If these mistakes are not corrected and they occur in several _____cell division control genes_ _, cancer may result. 3. 3. Which of the following would you most likely expect to find in a cancer cell? a p53 allele with a mutation which causes the substitution of 1 amino acid in the encoded protein, a hyperactive version of the APC protein, a growth factor receptor that cannot bind growth factor, or two alleles of DCC that both have nonsense codons 4. A linear piece of DNA has 3 EcoR1 sites. After digestion with EcoR1, you run your digest on an agarose gel, stained with ethidium bromide. How many fragments should you see? __ 4 ____ If the DNA was circular you would expect __ 3___ fragments. 5. What would happen to a plasmid if it got inside a bacterium but a)lacked an ori? it would not be replicated by the bacterium before cell division and not passed on to daughter cells B)lacked a selectable marker? the bacterium would spit it out since it would gain no advantage by keeping it 6. If a virus is able to infect both dog and human respiratory cells, then one can conclude that both cells contain almost identical ___ receptor proteins ______ 7. Viruses utilize the ribosomes of the host cell to translate their viral mRNAs because______ the virus does not carry its own .______ 8Would the following might make a restriction enzyme site in DNA I)5’ AAATTT 3’ yes it’s a palindrome II) 5’GGGGGGGCCC 3’ No, not a palindrome 9. You are trying to express human p53 protein in bacteria. You are inserting the coding region of the p53 gene into a plasmid at a multiple cloning site (sequence with several restriction sites) between a strong bacterial promoter and a bacterial transcriptional terminator. Why would it be better to insert the p53 CDS as an EcoR1/HindIII fragment instead of the same fragment with EcoR1 at both ends of the fragment? You need to
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