Linkage II - Building maps from 2-point cross data Genetic...

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Building maps from 2-point cross data Genetic maps Gene Pair R (%) Dist A, B 25 25 A, C 20 20 A, D 12 12 B, C 5 5 B, D 13 13 C, D 8 8 A, E 50 - A, F 50 - B, E 50 - B, F 50 - E, F 4 4
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Ordering close markers is a problem with 2-point crosses: order ABG or AGB? A B G 02 0 2 1 1 20 21 Gene Pair R (%) ABG A, B 20 A, G 21 B, G 1 AGB A, B 21 A, G 20 B, G 1 A G B 0 2 1 1 20 21 20 and 21 are very close. To distinguish 20 from 21 will require counting 160,000 flies
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Order ABG or AGB? Ordering close markers is much easier with 3-point crosses: 3-point Cross for mapping linked genes P1: AABBGG x aabbgg F1: AaBbGg = ABG/abg Testcross:
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This note was uploaded on 05/02/2008 for the course BIOS 221 taught by Professor Stone during the Fall '08 term at Ill. Chicago.

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Linkage II - Building maps from 2-point cross data Genetic...

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