Lesson 18 - 11\/1\/16 - Biotechnology Recombinant DNA Technology in Genome Manipulation DNA sequences from multiple sources can be recombined and inserted

Lesson 18 - 11/1/16 - Biotechnology Recombinant DNA...

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Biotechnology Recombinant DNA Technology in Genome Manipulation DNA sequences from multiple sources can be recombined and inserted into an organism Much faster method of trait introduction than classical selective based breeding Recombinant DNA technologies involve the manipulation of DNA using enzymatic reactions Biotechnology can be thought of as genetic engineering Use tools to manipulate DNA - enzymes, repurposed to fit our needs Restriction endonucleases Ligases DNA polymerase Genetic code = computer code Extract subprograms that can be re-incorporated into bigger programs - genes, promoters, miRNAs, etc. Cut and paste instructions from one organism to another Have to be able to analyze each subprogram Need file storage system to keep and store components Running the program requires a living organism Multipurpose techniques - PCR and electrophoresis PCR - amplification of a specific piece of DNA
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