{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Transformation-1101 - Biotechnology/Molecular Biology...

Info icon This preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Biotechnology/Molecular Biology Bacterial Transformation Biotechnology Branch of biology dealing with the study of DNA and genetics on the molecular level Biotechnologists manipulate DNA and genetic information to produce particular products or to study genetic disorders or diseases Medicine, agriculture, genetics, and the food industry Biotechnology Recombinant Technology Transformation: the placing of new genetic material into a cell changing the genotype and possibly the phenotype Vectors: entities that carry foreign DNA into the cell of an organism where DNA is expressed We will use a plasmid that confers antibiotic resistance to the transformed organism - E. coli cells Sterile Technique Don't open sterile packages until ready for use Avoid touching tip of pipet/transfer loop to anything Don't reuse tips prior to resterilization Keep sterile liquids covered Flame spreader and loop before/after each use Open petri dish only as needed, with it inverted when possible Deoxyribonucleic Acid http://www.accessexcellence.org/AB/GG/dna_molecule.html E. coli A gram-negative rod shaped bacteria found in the human intestine Has a single circular chromosome Can be easily cultivated and studied Grows rapidly and is highly used in lab settings Electroporation A small amount of electricity is applied to the cells in the presence of the plasmid This will create transient holes in the membrane which enable the cell to take up new DNA Cells that contain the plasmid (transformed) will be able to grow in the presence of ampicillin Micropipettes These tools can be used to accurately measure small amounts of liquid samples They come in a range of sizes and use tips, which provide sterility and prevent contamination Using Plasmids in Science Restriction Endonucleases Recognize a specific sequence and cleave the phosphodiester backbone of both strands of DNA at or near the recognition sequence Sequences are often palindromic ...
View Full Document

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern