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220914_WES_discussion_questions.docx - BF831 Translational...

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BF831 Translational Bioinformatics SeminarSeptember 26, 2022WES discussion questionsOVERVIEW1.What is the difference between whole exome sequencing (WES) and wholegenome sequencing? What are the advantages and disadvantages of each ofthese approaches?
2. How does next-generation sequencing (NGS) differ from Sanger sequencing? Whatis short read sequencing (e.g. Illumina HiSeq)? What is long read sequencing (e.g.PacBio SMRT)? What are the potential advantages/disadvantages of each approach?
3. Besides WES, what are other applications for NGS?4. What are the general steps (4 to 5) taken to analyze NGS data?
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