Chanal_et_al-2006-Environmental_Microbiology

Chanal_et_al-2006-Environmental_Microbiology - Blackwell...

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Environmental Microbiology (2006) 8 (3), 514–525 doi:10.1111/j.1462-2920.2005.00921.x © 2005 Society for Applied Microbiology and Blackwell Publishing Ltd Blackwell Science, LtdOxford, UKEMIEnvironmental Microbiology 1462-2912Society for Applied Microbiology and Blackwell Publishing Ltd, 2005 8 3514525 Original Article Diversity of Bacteria and Archaea in the desert of TataouineA. Chanal et al. Received 22 July, 2005; accepted 27 July, 2005. *For correspon- dence. E-mail [email protected]; Tel. ( + 33) 4 42 256 371; Fax ( + 33) 4 42 256 648. Both authors contributed equally to this work. The desert of Tataouine: an extreme environment that hosts a wide diversity of microorganisms and radiotolerant bacteria Angélique Chanal, 1† Virginie Chapon, 1 * Karim Benzerara, 1,2 Mohamed Barakat, 1 Richard Christen, 3 Wafa Achouak, 1 Frédéric Barras 4 and Thierry Heulin 1 1 LEMIR, DEVM, DSV, UMR 6191 CNRS-CEA-Université Aix-Marseille II, IFR 112, CEA Cadarache, F-13108 Saint- Paul-lez-Durance, France. 2 LMCP, UMR 7590 and Institut de Physique du Globe de Paris, 4 place Jussieu, F-75252 Paris, France. 3 UMR 6543, CNRS – Université de Nice Sophia Antipolis, Centre de Biochimie, Parc Valrose, F-06108 Nice, France. 4 LCB, CNRS, 31, chemin J. Aiguier, F-13402 Marseille, France. Summary The phylogenetic diversity of prokaryotic communi- ties exposed to arid conditions in the hot desert of Tataouine (south Tunisia) was estimated with a com- bination of a culture and – molecular-based analysis. Thirty-one isolates, representative of each dominant morphotypes, were affiliated to Actinobacteria , Firm- icutes , Proteobacteria and the CFB group while none related to Archaea. Analysis of 16S rRNA gene librar- ies revealed the presence of species related to Bac- teria and Archaea . Sequences related to Archaea were all affiliated to the non-thermophilic Crenarchaeota subgroup. Bacterial sequences were dominated by Proteobacteria , Actinobacteria and Acidobacteria ; a few sequences were distributed among eight others phyla, including Thermus/Deinococcus relatives. A correlation between tolerance to desiccation and to radiation has been demonstrated for the radiotolerant bacteria Deinococcus radiodurans. Because bacteria living in the hot desert of Tataouine are one way or another tolerant to desiccation, we investigate whether they could also be tolerant to radiation. Expo- sition of soil samples to intense gamma radiation yields Bacillus , Thermus/Deinococcus and α - Proteo- bacteria relatives. Four of these strains correspond to radiotolerant species as revealed by evaluation of the resistance levels of the individual cultures. A detailed analysis of the resistance levels for two Thermus/ Deinococcus and two α - Proteobacteria relatives revealed that they correspond to new radiotolerant species. Introduction Living organisms in hot arid environments have to deal with unfavourable life conditions, including dryness, extremes of temperatures, strong thermal contrasts and lack of nutrients. The different ways of how bacteria resist to these stresses are largely unknown. Approach rests on the phylogenetic analysis of communities inhabiting arid
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  • Fall '16
  • Statistics, Bacteria, 16S ribosomal RNA, Society for Applied Microbiology, Tataouine, Uncultured bact

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