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AsepticGermTheory_S16 - Biology 151 1 LABORATORY MODULE 2...

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Biology 151LABORATORY MODULE 21Aseptic Technique & Germ Theory of DiseaseAll living cells share many biochemical and physical similarities; however not allcells are equivalent.Prokaryoticcells do not contain a membrane-bound nucleus like theeukaryotes you have seen.Instead their DNA is suspended in a portion of the cytoplasmcalled thenucleoid.Prokaryotes are also typically much smaller than eukaryotes.Eukaryotic and prokaryotic cells exhibit other important structural and chemicaldifferences.Many of these differences determine the role of prokaryotes in nature, fromrecycling nutrients to causing disease in humans.The differences between eukaryotic andprokaryotic cells also provide the basis for treating diseases caused by bacteriaGrowing MicroorganismsTo culture microorganisms or any cell type, a medium containing essential nutrientsand specific incubation conditions for maintaining life is necessary.Bacteria can bemaintained in culture in either a liquid media or on a solid media containing agar - agelatinous material derived from certain marine algae used as a base for bacterial culturemedia. Some media such asTSA(Trypticase Soy Agar) orTSB(Trypticase Soy Broth) willsupport the growth of most types of bacteria.Aseptic Techniques – Some General Rules1. Disinfect the bench top with an appropriate disinfectant before you begin working andafter you are finished with the experiment.2. Work on a clear bench top.Put away all unnecessary items.3. Wear the necessary personal protective equipment (lab coat, gloves, goggles, etc)4. Wash your hands before and after manipulating a sample.5. Keep all cultures closed and tubes upright in a rack until ready for use.6. Hold cultures by the tube NOT the cap.7. Work quickly and carefully - don't become distracted or allow others to disturb you.8.Waste Disposala. Contaminated paper & plastics go in the biohazard trash bin.b. Contaminated glass (with or without bacteria) NEVER goes into the biohazardtrash bin – it goes into the contaminated broken glass bin in the fume hood.c. When in doubt – ASK!
2LABORATORY ACTIVITY 2What is Aseptic Technique?Most experiments performed using microorganisms usesterilemedia andpurecultures(progeny of a single organism).A series of operations have been developed tolimit the risk of contaminating these materials during manipulations.These operations areknown asaseptic techniques.They also help protect investigators from infectingthemselves or releasing the organisms into the environment.Learn the following rules andprocedures and understand how each requirement contributes to maintaining asepsis (stateof being free of living pathogenic microorganisms).The Tools for Aseptic Transfer1.Microbes can be removed frombrothwith a sterileinoculating loop,serologicalpipet,or amicropipettorwith sterile pipet tips.Microbes grown onsolid mediacan be removed with a sterileinoculating loop, inoculating needle, or toothpick.

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Term
Spring
Professor
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Tags
Organic chemistry, Bacteria, Nucleus, pH, loop, Agar plate, Petri dish, Aseptic Transfer, Microbiological culture

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