25 - Bio Recombinant DNA I 2/25/2008 10:12:00 AM 1)...

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Bio – Recombinant DNA I 25/02/2008 10:12:00 1) Cleavage of DNA at specific site using restriction enzmes 2) Electrophoresis 3) Making copies of DNA using polymerase chain reaction 4) Rapid Sequencing of nucleotides 5) Nucleic acid hybridization 6) DNA cloning – millions and billions 7) DNA engineering – alternation of DNA sequences to modify genes Restriction enzymes Bacteria in origin Cut up foreign DNA Restriction endonucleouses Cut DNA at very specific points “Recognition sites” o 4-8 base pairs 100 different kinds CONSISTANT – find the same recognition site, same cuts, and same  fragments. However different enzymes cut at different places and give you  different fragments. Staggered cut or straight cut 5’ G | AATTC 3’ 3’ CTTAA | G 5’ _____G AATTC_____             AATTC_____ _____CTTAA
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This note was uploaded on 03/03/2008 for the course BIO G 102 taught by Professor Walcott during the Spring '08 term at Cornell University (Engineering School).

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25 - Bio Recombinant DNA I 2/25/2008 10:12:00 AM 1)...

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