Demo Lab 4 - Demo Lab 4 Katie Bredice Wednesday Section...

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Demo Lab 4 Katie Bredice Wednesday Section Henry Wen Optical Tweezers 14 November 2007
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In this lab we demonstrated the use of Optical Tweezers. In general, a laser beam is focused by a high-quality microscope objective to a spot in the specimen plane. This spot creates an "optical trap" which is able to hold a small particle at its center. The forces felt by this particle consist of the light scattering and gradient forces due to the interaction of the particle with the light. We filled a slide with tiny polystyrene balls and looked at them through a microscope in order to trap one single ball in place using a laser and drag it around. The setup consisted of a laser, beam enlarger, weak focus, strong focus, the microscope and a camera to view the slide. When we turned the knob on the microscope it showed the different planes going in and out of focus. When the laser entered the balls seemed to slow down and gravitate toward the trapped one, which showed three-dimensional trapping. When the bead is displaced from the beam center, the
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