CHEN 1000 Biotech Lecture 9 Notes

CHEN 1000 Biotech - Announcements Exam#2 Location Tuesday 3/18 7 PM Either Math 100 or Coors The location will be announced on CULearn and by email

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1 Announcements Exam #2: Tuesday 3/18, 7 PM. Location: Either Math 100 or Coors. The location will be announced on CULearn and by email on Monday evening 3/17. Prof. Schwartz will have special office hours next week. Monday 9:30-11 Tuesday noon-2 (no Wed or Thurs office hours) Practice problems are posted for Exam #2 Other Applications of Biotech Tools Uses of DNA Chapter 9 of Understanding Biotechnology DNA Fingerprinting - Every individual has a unique set of DNA (except identical twins) - Human genome = 3,200,000,000 nucleotides - At 99.99% identical, 320,000 differences remain! • Used to characterize biological samples for legal proceedings; to “match” sample DNA with DNA taken from a defendant or to rule out the possibility of a match. • Also used for paternity testing since the DNA fingerprint of a child is a composite of the patterns of the mother and father. Making a DNA Fingerprint (1) Digest sample DNA with a restriction endonuclease . This results in a distribution of fragments with various sizes. (2) These fragments are separated by size by placing them on a gel exposed to an electric field that forces them to move. Smaller fragments move through the gel more easily and travel farther. (3) Images of the gels show distinctive “bands” that indicate the sizes of the fragments that represent the distinctive distances between restriction enzyme binding sites. The resulting image is like a “histogram” of fragment sizes. DNA Fingerprint Bands small fragments large fragments Clicker Question Is the alleged father the true father? a)Yes b)No The DNA fingerprint of a child is a combination of the mother’s and father’s fingerprint.
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2 Clicker Question What enzyme could be useful if you wanted to duplicate a piece of DNA? A: RNA polymerase
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This note was uploaded on 05/01/2008 for the course CHEN 1000 taught by Professor Degrazia,j during the Spring '04 term at Colorado.

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CHEN 1000 Biotech - Announcements Exam#2 Location Tuesday 3/18 7 PM Either Math 100 or Coors The location will be announced on CULearn and by email

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