CHEN 1000 Biotech Lecture 13 Notes

CHEN 1000 Biotech Lecture 13 Notes - Molecular Recognition...

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1 Molecular Recognition Many molecules are attracted to each other, e.g. water molecules are attracted to other water molecules, oil molecules are attracted to other oil molecules. Biomolecules like DNA, RNA, and proteins often have very specific attractive interactions that are present only when a particular pair of molecules encounter each other. DNA Hybridization DNA interactions are conceptually simple: complementary sequences attach, or hybridize . ---GCCTTAGCACGGAATTGAACCGGC--- TCGTGCCTT + small DNA probe ssDNA TCGTGCCTT ---GCCTTAGCACGGAATTGAACCGGC--- Protein-Ligand Interactions The binding pocket of a protein is shaped specifically to recognize and hold a certain molecule. protein ligand binding pocket • An enzyme recognizes a substrate molecule to cause a chemical reaction • An antibody recognizes and binds an antigen to cause an immune response. Antibody Antibodies produced in vitro can be used . .. •to diagnose pathogens (immunological diagnostics) •to target disease cells for drug delivery How is it made? Produced by B-cells in response to contact with a foreign object/substance called an antigen . What is it? Protein with a binding pocket that recognizes a subunit (part) of the object. How does it work? Binds the object and sends a signal to the rest of the immune system. Clicker Question An important characteristic of antibodies used for diagnosis is that they do not accidentally recognize the wrong object. This property might be called . .. A: strong binding B: universality C: specificity Polyclonal Antibodies A typical virus will have multiple potential recognition sites. So an organism may produce a number of antibodies.
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CHEN 1000 Biotech Lecture 13 Notes - Molecular Recognition...

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