ch 20 - Chapter 20 DNA Technology and Genomics MULTIPLE...

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Chapter 20: DNA Technology and Genomics MULTIPLE CHOICE 1) Plasmids are important in biotechnology because they are A) a vehicle for the insertion of foreign genes into bacteria. B) recognition sites on recombinant DNA strands. C) surfaces for protein synthesis in eukaryotic recombinants. D) surfaces for respiratory processes in bacteria. E) proviruses incorporated into the host DNA. ANS: A PTS: 1 TOP: Concept 20.1 2) If you discovered a bacterial cell that contained no restriction enzymes, which of the following would you expect to happen? A) The cell would be unable to replicate its DNA. B) The cell would create incomplete plasmids. C) The cell would be easily infected and lysed by bacteriophages. D) The cell would become an obligate parasite. E) Both A and D would occur. ANS: C PTS: 1 TOP: Concept 20.1 3) Which enzyme was used to produce the molecule in the figure below? A) ligase B) transcriptase C) a restriction enzyme D) RNA polymerase E) DNA polymerase ANS: C PTS: 1 TOP: Concept 20.1 4) Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation of genomic DNA that has been cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to A) insert the fragments cut with X directly into the plasmid without cutting the plasmid. B) cut the plasmid with restriction enzyme X and insert the fragments cut with Y into the plasmid. C) cut the DNA again with restriction enzyme Y and insert these fragments into the plasmid cut with the same enzyme. D) cut the plasmid twice with restriction enzyme Y and ligate the two fragments onto the ends of the DNA fragments cut with restriction enzyme X. E) cut the plasmid with enzyme X and then insert the gene into the plasmid.
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ANS: C PTS: 1 TOP: Concept 20.1 5) What is the enzymatic function of restriction enzymes? A) to add new nucleotides to the growing strand of DNA B) to join nucleotides during replication C) to join nucleotides during transcription D) to cleave nucleic acids at specific sites E) to repair breaks in sugar-phosphate backbones ANS: D PTS: 1 TOP: Concept 20.1 6) How does a bacterial cell protect its own DNA from restriction enzymes? A) by adding methyl groups to adenines and cytosines B) using DNA ligase to seal the bacterial DNA into a closed circle C) adding histones to protect the double-stranded DNA D) by forming "sticky ends" of bacterial DNA to prevent the enzyme from attaching E) by reinforcing the bacterial DNA structure with covalent phosphodiester bonds ANS: A PTS: 1 TOP: Concept 20.1 7) What is a cloning vector? A) an enzyme that cuts DNA into restriction fragments B) a DNA probe used to locate a particular gene in the genome C) an agent, such as a plasmid, used to transfer DNA from an in vitro solution into a living cell D) the laboratory apparatus used to clone genes E) the sticky end of a DNA fragment ANS: C PTS:
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This note was uploaded on 05/01/2008 for the course BIO 320 taught by Professor Walsh,weinert during the Spring '07 term at Arizona.

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ch 20 - Chapter 20 DNA Technology and Genomics MULTIPLE...

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