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Unformatted text preview: Arria Owlia apo223 Bio 206L Lloyd 50105 01/29/08 1:30 PM Lab 1 Fluorescence Microscopy Purpose: This lab helped students to become acquainted with the many advantages of fluorescence microscopy over standard microscopy. Abstract: The first item in our procedure was to observe beads prepared by the TA. We were to get a feel for the illumination shutter, the filter cubes, the light source, and the fluorescence microscope in general. After completing this step, we were to create epithelial cell stains using Propidium Iodide and DAPI. These agents stain the nuclear portions of the cells. This showed us how to distinguish between brightfield and fluorescence microscopy. Next, we prepared L8 cells that should have been actively dividing. We were to observe the cells under different conditions: phase, with DAPI, with DAPI and rhodamine phalloidin, with DAPI and fluorescein. Upon completion of this last procedure, we had a far greater DAPI and rhodamine phalloidin, with DAPI and fluorescein....
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- Spring '08