PCR[2] - Polymerase Chain Reaction By Kurt Scavelli and...

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Polymerase Chain Reaction By Kurt Scavelli and Neil Mehta
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Founder- Kary Mullis Invented PCR method 1983 Noble Prize Chemistry 1993 Purpose for development: Formulate a process by which DNA could be artificially multiplied through repeated cycles of duplication driven by an enzyme called DNA polymerase.
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Overview Basic Overview: (1) Denaturing at 94-96°C. (2) Annealing at (eg) 68°C (3) Elongation at 72°C (4) The first cycle is complete The two resulting DNA strands make up the template DNA for the next cycle, thus doubling the amount of DNA duplicated for each new cycle
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Materials Thermal Cycler DNA Template 2 Primers Taq Polymerase Deoxynucleotide Triphosphates (dNTPs) Buffer Solution Divalent Cation Monovalent Cation
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A specific sequence is selected to be amplified(red). This sequence can be any gene of interest or a non-coding portion of DNA. In order to copy the gene, a short
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PCR[2] - Polymerase Chain Reaction By Kurt Scavelli and...

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