Exam 4 -2005

Exam 4 -2005 - NAME You may pick up your exam from...

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NAME_________________________ You may pick up your exam from Professor Hinkle next semester or, if you wish, we will put your exam in the hall (next semester) near professor Hinkle's office (HH 310). Please put my exam in the hall _________. BIO 198 GENETICS FINAL EXAM December 18, 2005 200 Points 3 hours (~1.1 Points/Min) Instructions : 1) Write your name at the top of each page. 2) Answer questions, in the space provided, using ink . If you change an answer, clearly cross out the information you do not wish to be considered by the graders. Do NOT use white-out. 3) Please write neatly and be concise. Use pictures or diagrams if appropriate. 1)_______/15 2)_______/24 3)_______/22 4)_______/34 5)_______/36 6)_______/22 7)_______/23 8)_______/24 TOTAL________/200
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NAME_________________________ 1. (15 points) Very briefly define each of the following terms: a) HAT (hint: chromatin) b) insulator (hint: chromatin) c) histone tail d) EcoRI linker e) cDNA library
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NAME_________________________ 2. (24 points) a. (6 points) Explain the difference between a genetic screen and a genetic selection. b. (10 points) Briefly describe the genetic screen used by Clark to find the E. coli Rec - mutants. c. (4 points) What other property (phenotype) of the Rec mutants indicates a role for recombination in DNA repair? d. (4 points) What is a RecA filament?
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NAME_________________________ 3. (22 points) Yeast UV sensitive mutants are called Rad mutants. (Sensitive to UV rad iation) You have isolated two such mutants, rad -1 and rad -2, which each show the same increased sensitivity to UV radiation. a. (5 points) When each mutant is crossed with a wild type Rad + yeast, the resulting diploids are all Rad + . What does this result mean? b. (5 points) When the two mutants are crossed with each other, the resulting diploid is also Rad + . What does this result mean? c. (6 points) When the diploids in “b” are sporulated, after germination most of these spores
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Exam 4 -2005 - NAME You may pick up your exam from...

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