BIO 198 Exam 2 - 2007 Key

BIO 198 Exam 2 - 2007 Key - Statistics for Exam 1! Median...

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Unformatted text preview: Statistics for Exam 1! Median 68 Mean 66.7 High 93 Low 23 Calculated as a percent of total points {Exam 1 + Exam 11 + homework to this point =18? pts): 80 & above A ?5—?9 A- 70.74 B+ 65—69 B 60-64 B- 55-59 C+ 50-54 C 45-49 C- 40-44 D+ 35-39 D 30-34 D- 29 & below E NAME 1. ([0 points) Define the following terms: a. Co-dominance W!" A‘ kt‘mfll’; Vtk?mm bahfi- tau-Jub- ck. A‘BD bunt '59:: I+ crud-£5 a have 90.9.1959“, mafia. mix. "lu ‘Ebf 4 o'clock: Planar “M?L¢ b. Auxotrophy A gi-raun ig__vhfi.bkf_ 1:1; 5mm om mrmnt Maul-baa q- "gluing: 4! mm - 4' gawk-ad “ Testy-(fan 5v?ka 4'9 gran-u {F 3- Synthetic lethal Unit-ch. *- JM fin unhml m Aha-n. “6“”- I do};ch mul-ml- rs l-L'HAA'I’ 5:3” mitt-rm: MM‘FLhmam waist-a). *I 4' deal!- kAu-c {1, M?lam “nai- M4 muuhm, MM‘E Lam: FF (ah-u Lq=3=3 .( 2”.) rs 5w“- d. Molecular markers & 511-; A 'DNA mepgb{fi¢-LP%, Sufi-’3, “5112(3) *ku— cn-m br. uycd as q. J-fi/rt‘FWM-‘a. F‘mfli“ e. Heterokaryon 1Q" Cg,“ calm a; 4190 OliFFCrf-n" h‘dCLC-i Rh. 9.. Jknw—tc). JI 5w 4:419:qu hack: 4'1- 8, 9% BWKW or "6416“ 2. (18 points) a. (9 points) You have isolated four different mutants unable to synthesize some compound E for growth. You know that compounds A through D are required in the biosynthetie pathway, but you do not know the order in which they are synthesized by a wild—type cell. You test each of the following growth products for its ability to support the growth of each mutant. Mutant A B C D E l — — — - + 2 — +-. + + + 3 w — + + + 4 7 —- + — + Using the data above; i. (5 points) construct a biochemical pathway indicating the order of the compounds. 4 y 4r 3 ,_ A F- e—a e C H is w . _ . IJ‘ "r\\ I": .__II\'\\\~. \_-."."\\ ii. [4 points) on this map, indicate where each mutant is blocked b. (9 points) Suppose that in your lab you occasionally get mine that have bent ears. You determine that the bent-eartrait is recessive. You then find out that someone else in your lab has isolated mice with bent ears as well and that they have also determined that bent ears are a recessive trait. You cross one of your bent—cared mice with one of your lab mate‘s bent-eared mice. All of the progeny hava normal ears. The ratio you get from crossing the F15 is 9:7 (normal to bent). i. {1 point) What type of test have you performed? NAME W 3. (17 points) a. (13 points) In Neurospora, a strain auxotrophic for thiamine (mutant allele 1) was crossed with a strain auxotrophic for methionine (m). Linear asci were isolated and sorted into the following classes. (To simplify this analysis, we are ignoring the post-meiotic division). i. (6 points) On the lines below each ascus type indicate whether it is parental ditype (PD), non— parental ditype (NPD), or tetratype (T) with respect to the thiamine and methionine loci. 1 2 3 4 5 6 t + t + t + t + t m t m t + t m + m + + t m + + \ } Wm + m + + t + t m + + t + Q + m + m + m + m + + + m 260 76 4 54 l 5 PD r PU (fl; ii. (3 points) Are the genes Tand Mlinked? Explain how you came to this conclusion. n X y W NPD 4 PA“ iii. (4 points) Calculate the distance in map units from Tto its centromere. 7; I F L4H >£ ’ + S A W L‘ l", _ 'i‘ ) :_ K /00% :— Z ? 7a PJVWQ ? Z¢mw b. (4 points) In Drosophila, the two genes fl and ok are X-linked and 14 map units apart, A female fly of genotype fz+0k+/fz 0k is crossed to a male from a wild—type line. What percent of male progeny will be fz+ok? . / 2, '7 4. (25 points} a. (5 points) For each statement, indicate which mechanism of gene transfer applies: Tran orrnation (Ti), Conjugation (C), or Transduction (Td) Bacteriophages are required for gene transfer 73 DNA is acquired directly from the environment if, p The movement of DNA is unidirectional from P to F 6” The transferred DNA must reco hine and replace the DNA in the chromosome in order to be maintainedm 9011: Le: 1‘ €93. Til—Dr It can be used to study the dominance relationship between alleles via the creation of partial diploids b. {6 points) You have two F' bacteria that are atrxotrohic for methionine. Strain 1 is met‘; Strain 2 is met' and defective in recombination. i. (3 points) If a Met+ Hfr strain, in which the origin of transfer is only 5 minutes from the met locus, was allowed to conjugate with Strains l and 2 for 20 minutes. Exoonjugants were grown on minimal medium to select Met+ cells. For each strain would you get many Met+ colonies or few? In one sentence explain why. 4 .fi' . - 1 at... 1: w an 4’ LS-Mkt'f'l 311 1&4... . _ ‘ ‘I Liam- ML{‘L<S‘1GJ.IIHII CD: CU ' m [athlJ'mJicn I‘Sf‘tt-SS‘KJizi , (clad—A ‘1 fl i 2 ll" t? nei- l‘AL-Q L“; (h lfififlwg VF H LglierLW‘fiC‘ . l i. (Llr - l E I I l + “F” 1446’ {MM RLJ—ré'fr'fierhjv.j1iv-A"l Q )5“ "l KY5 h! In 4A" {mu 0% {ECG-‘14” ‘lfihfl mrns. _ ‘ ii. (3 pointsl manila: experiment was performed, except that an Famer-i: strain was used as the donor. For Strain l and Strain 2, would you get many Met+ exconjugants or few? Again, explain why. ' I 1 -. . 1 .. . . . _ - - _l- l. — (5“il‘f'L‘chfi lsx *— fir F— rr.‘\{ reflux (LI, LLm-luuq tori U; _ K ' 'e . - i l'\ 'é 9.411;; :L’SU"“(I {LL-{fl ##r Q7573: '13. {NJ (J 4L." -IS.‘C\|'\-£ .. 4A 6 r. Cd‘nr . __ {rub-E #3 ‘ LU“ Alla W] l- and r u: mil. agent‘s. rib-J 5 l -* r V“! (dike/l *3 m “I ML} 4414 nan" Cal/5,, {:1.r"i?lir'tj .1 -_~ Elg‘f'”: 551) . nJLEfi Lg NAME E a Question 4 continued 4c. {4 points) List two differences between generalized transduction and specialized transduction. Ii) Road-3““ &/ j [vb—F‘- 1) S w Js l‘“"f-“-""' W’Wd In (T d. (6 points) A transduction experiment was conducted using a prototrophic donor and a recipient that was auxotrophic for Iys, arg, and HP. Trp+ transductants were selected. The Trp" transductants could be categorized into the following classes: Genotme Number ly s‘arg' l0 lys'arg‘ 13 l-yslargL ————32 — lys‘arg‘ l i. (3 points) Which marker is closest to trp‘? Explain your reasoning. ii. (3 points) What is the order of the three markers? Explain your reasoning. . +7 "1V ] fi-‘chucx AZ (2,13 Lei-u- f;k-./?’L--t-.-c—-—;.—- L’ .__l.—-—l——l———- . ‘ -- . \ . . ,, - . l-LY‘ «HP {LY-".3; :' 4).? (C; (TI—Lt" f; L« /7fl"_ {LT—‘4’! {cc/:4: /3¢' (ti-7(6— #— )2 . a...) 3 Lb: II L UL .Lc‘ w 4J1. , h, . ad " '1' I \U L , at .__ 0/913» 3‘0 )‘V‘g \%®__hLLh iv. ‘b, for» but” a UL-vi say! hf /\ [03" 9;” L1- uv'U 'l. E) “I”? if \ L1 ll 96 fi’tcj Sc-‘fl e. (4 points) Temperate hage like A form turbid plaques on a lawn of bacterial cells. explain hyiri'l a Wail . I . . -- . f c :' K‘ '7 7‘2... éKZédc-w 7431/:«fi/Ckr‘6w0 AMA-1% 7/ /)/é - r ‘ - " 17432.3(: 464». 2‘7? 3,! :. .?__ 6”." 9’ gflfa-‘r’ [3:- _ S /t’/£'Ul£-(' .4 0‘7Hffl-56 K“? C7 ‘7’"75 QW7W‘1- (“i-t— yk "4 3 (1/ rag—'1' C 9"5'7‘47LK ADJ/21%; Mic” c641"; _ (,3, z 1 H x - :' ' I'- ' ' 4 .-, z. _ If?! . 7n 5‘ /~(z’t ,i réf/rwt'mj ([1 g” " HE J I} I “I. U. .U‘. fix)”; _ - - ' x’} ' ".1. ILA. " f?- (r’mm’wa7 J— 2 44 exit -" i NAME 5. (30 points) a. (5 points) Draw the structure of 2 deoxyribonucleotides connected by a phosphodiester bond using the stick figure notation. Clearly label the 5’ and 3’ ends. glbtib , [at 5 / F'P'f\/p on,” b. (18 points) Draw a prokaryotic replication fork. On this figure, indicate leading and lagging strands, 5‘ and 3‘ ends, and indicate the following proteins (in a few words. describe the function of each protein in the following list): i. DNA'Polymerase III N ii. B-clamp rl iii. ligase 4 1 ff: iv. DNA Polymerase I 11. v. DnaB {helicase} *' vi. DnaG (prirnaseli'1 A .. \ "' VILSSb'I @‘I K? :25, “3&5” 1 .l- 'L‘ (1" k 1 r .' , he”? fi lame t‘ ‘ /'§ tar/Hm 2- A ~- ' o . fl - +I 5‘ 5 IIII - 3' @151.in $i1.:Lfllfl - gltxfifin mummm 01 ptl-‘flIz ’f H;- DNA Pci.]]I- gxml-l’lcg'té m 9—) Zn itlrfmm; 3L.» 6' emailing-mmtry E‘IHU’YIp— htldr: pol. IE on We DNA (701W: mL-rt tiht‘lthf "4“‘95 ‘ 1mm: Mo mnitchdte to inrmmq phttopmmwrur tar-rid blw 3'DH ? 9‘P DNA P“ I ‘ WWW-59'“? "’1 6' —> ’5' ttlvtmm; FLPthwlmuUtfléfi» (9"??? N); 3*? 6' e xomutlmoo amt.“an (wounding) DNA Behellmofi -— unwmdo DNA Uncle: {pmmeG— mile nuwn P—NA pvt/imam l’tr’ gatjmheele 11' rpm” See {emit gnawed hmAIl’lg Miami-19mm; 17.- Al.va in. m ’r ff 1' 117 a“ NAME £h%”~“£[ } = Question 5 continued ' 5c (7' points) Briefly describe how DnaA promotes replication initiation in E. coir“. q r - r-' \<__.\: Q“? w mambe (egg-bqu 7 _ 1.. . . n 'r‘ ' flunk 4115'“ EMT-qu *5 3Y0 15kt} Ljfi‘kfll‘d (mth 3 “lie;qu [H with QM“. 3% 1:2 I.L"r'\LQELLt‘.’-.fl\ ...
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This test prep was uploaded on 04/17/2008 for the course BIO 198 taught by Professor Sia during the Fall '08 term at Rochester.

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BIO 198 Exam 2 - 2007 Key - Statistics for Exam 1! Median...

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