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hemoglobin - Electrophoresis of Hemoglobin Hemoglobin A red...

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Electrophoresis of  Hemoglobin
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Hemoglobin A red pigment found in Erythrocytes (Red Blood  Cells – RBCs) Binds directly to Oxygen which allow the RBCs to  transport and delivery oxygen to cells  Each RBC contains about  280 million Hemoglobin  molecules    Mature Erythrocyte For every 1 mL of blood there are about 5 billion  RBCs
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The RBC will pick up  Oxygen (O 2 ) from the lungs  and will release Carbon  Dioxide (CO 2 ) to the lungs. CO 2  is a major by-product  of metabolic activities that  occur in a cell. The amount  of CO 2  produced by a cell  and the demand for O 2   is  directly proportional to its metabolism. 
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Hemoglobin (Hb) is made of 4 polypeptide  chains:    2  β  (Beta) (Identical  β  strands)  2   (Alpha) (Identical   strands) On each  of these 4 chains is a bound  Heme Group :   Consist of a  Porphyrin (Nitrogen)  Ring with and Iron (Fe) atom at its  center
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The different Hemoglobins we will be analyzing  are the following:     1.  HbA  Accounts for 98% of total Hb in normal adults  Consists of:   - 141 amino acids (aa)
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