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P1 and P2 maps - Plasmid pAMP EcoRl Nari 4368 Ol4539...

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Unformatted text preview: Plasmid pAMP EcoRl Nari 4368 Ol4539 E0001091 580“ 4133 amp’: ampioillin resistance gene Origin: origin of DNA replication Lambda.DNA sequences: 0 - 1904 bp pUC19V DNA sequences: 1904 - 4534 bp M/ul994 BamHl1120 BGlll 1199 amp’ pAMP 4539 bp Origin ‘9 Hindl|l1904 Restriction sites are shown for selected enzymes that cut the plasmid only once. Poly cloning 10 Kb site Hind m Eccfii pucns/pucns (3.2 kb) Em H. INSERT” ~60 Kb puc 119 3.2 Kb Hind ||| *lnsen was digested with Hind Ill lens i 2 : . 1 2 a 4 s s 7 e n m u i: 1: u as u a m 5 i r I rm Mn n. m m Sn L” um All Dy: m 564 m Llu Glu Ann in. Am vm Pra 5m s« A." 5-1 u» A:- no ACC no A" ACG on As: we :AY sec TGC A86 vac ACT cu GAG an CDC cm: on use As: was MT YEA c'G GCC | L l 1 l. J l l l 1 l l ‘ l J l Hmmu 5pm Fm Sail xzm Ban-1H1 Smut Kpni Sm Econ! Accl )(nul Nam! ...
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