agarosenoflowchart

agarosenoflowchart - Agarose Gel Electrophoresis...

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Unformatted text preview: Agarose Gel Electrophoresis Electrophoresis: It is the separation of a mixture of charged particles in the presence of an electrical field. Examples of Different Types of Electrophoresis: Cellulose Acetate Electrophoresis Polyacrylamide Gel Electrophoresis (PAGE) Agarose Gel Electrophoresis Migration of these charged particles depend on 3 factors : 1.) Applied Voltage 2.) Molecular Friction (Sieving) 3.) Net Charge Agarose at Room Temperature is a 3-Dimentional solid matrix. This solid matrix will allow the separation of fragments by size. The smaller the fragments the further the migration or movement through the matrix. The greater the concentration of agarose, the tighter the matrix. Charged Fragment B Charged Fragment A 3-D Agarose Matrix Cathode Anode A B Loaded Wells 1% Agarose Gel The distance traveled by these charged fragments is inversely...
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agarosenoflowchart - Agarose Gel Electrophoresis...

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