Agarose - Agarose Gel Electrophoresis Electrophoresis It is...

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Agarose Gel  Electrophoresis
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Electrophoresis: It is the separation of a mixture of charged particles in the  presence of an electrical field. Examples of Different Types of Electrophoresis:  Cellulose Acetate Electrophoresis  Polyacrylamide Gel Electrophoresis (PAGE)  Agarose Gel Electrophoresis Migration of these charged particles depend on  3 factors : 1.) Applied Voltage 2.) Molecular Friction (Sieving)  3.) Net Charge
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Agarose at Room Temperature is a 3-Dimentional solid matrix. This solid matrix will allow the separation of fragments by  size. The smaller the fragments the further the migration or  movement through the matrix. The greater the concentration of agarose, the “tighter” the  matrix.      Charged  Fragment  B    Charged Fragment  A    3-D  Agarose   Matrix Cathode Anode A B  Loaded   Wells 1% Agarose Gel
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This note was uploaded on 05/03/2008 for the course BIO 2322 taught by Professor Spotswood during the Spring '08 term at The University of Texas at San Antonio- San Antonio.

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Agarose - Agarose Gel Electrophoresis Electrophoresis It is...

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