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isolatdroso - Isolation of DNA from Drosophila using PCR...

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Isolation of DNA from  Drosophila  using PCR
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PCR: P olymerase  C hain  R eaction (PCR) PCR is a simple, quick method for making many copies of a  specific segment of DNA without using cells (in vitro).   It is extremely important because of its sensitivity , specificity and speed It has revolutionized genetic analysis and detection of disease  pathogens. For example, PCR is used to identify specific  disease-causing mutations (e.g., Sickle Cell Anemia,  Huntington’s disease) The whole amplification process takes place in a PCR machine  that undergoes several cycles (Automated Thermal Cycling) to  generate the many segments of DNA. 
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Advantages and Limitations of  PCR: Advantages:  Only a very small amount of DNA (1ug or less) is  needed for PCR.  Results are achieved in less than a few hours or less  than a day. Limitations:  Sequence information must be available to synthesize  the primers that are essentials for PCR.
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