isolatdroso - Isolation of DNA from Drosophila using PCR...

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
Isolation of DNA from  Drosophila  using PCR
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
PCR: P olymerase  C hain  R eaction (PCR) PCR is a simple, quick method for making many copies of a  specific segment of DNA without using cells (in vitro).   It is extremely important because of its sensitivity , specificity and speed It has revolutionized genetic analysis and detection of disease  pathogens. For example, PCR is used to identify specific  disease-causing mutations (e.g., Sickle Cell Anemia,  Huntington’s disease) The whole amplification process takes place in a PCR machine  that undergoes several cycles (Automated Thermal Cycling) to  generate the many segments of DNA. 
Background image of page 2
PCR: Advantages:  Only a very small amount of DNA (1ug or less) is  needed for PCR.  Results are achieved in less than a few hours or less  than a day. Limitations:  Sequence information must be available to synthesize  the primers that are essentials for PCR.  Limits to the number of copies that can be made, 
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 21

isolatdroso - Isolation of DNA from Drosophila using PCR...

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online