17_Restriction_Digestion__LAB_17_ - Restriction Digestion...

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Unformatted text preview: Restriction Digestion Restriction Enzymes (RE): Bacterial proteins that protect the bacteria’s DNA from incoming foreign DNA (e.g. viral DNA). These are endonucleases that cut DNA based on recognition of specific, short DNA sequences called Restriction Sites . Whether a given Restriction Enzyme is able to cut DNA depends on 2 factors: 1. The existence of the specific, short DNA sequence that the particulare enzyme recognizes. The specific short sequence can be 4 , 6 or 8 base pairs . 2. The state of methylation of the recognition sequence in the DNA to be cut. Only unmethylated DNA will be cleaved . Methylation is done by an enzyme called Methylase . How Restriction Enzymes are Named: EcoRI The first letter corresponds to the GENUS of the bacterium (e.g. Escherichia ) Next two letters corresponds to the SPECIES of the bacterium...
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17_Restriction_Digestion__LAB_17_ - Restriction Digestion...

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