19_SouthernBlot_Lab19_ - Southern Blot Southern Blot: It is...

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Southern Blot
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Southern Blot: It is an analytical technique used to detect the  presence and state of DNA sequences. There are 2 other types of Blots: 1.  Western Blot :  Used for protein sequences 2.  Northern Blot :  Used for RNA sequences
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Southern blot involves the transfer of DNA  from the gel on to a membranous sheet. 2 Types of Membranous Sheets: 1.  Nylon Membrane 2.  Nitrocellulose Membrane We use the   Nylon Membrane for the following reasons:  Durability  Binds DNA < 500 b.p.  Ability to be Reprobed  Expensive Such a transfer will  immobilize the DNA stabilize the DNA and make the  DNA accessible to a probe . Before the transfer, the DNA must be made  Single Stranded .
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Important Solutions used in Southern Blot: 1.     Denaturing Solution  (1.5M  NaCl  + 0.5M  NaOH ):  This alkaline solution will denature or “melt” the double 
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This note was uploaded on 05/03/2008 for the course BIO 2322 taught by Professor Spotswood during the Spring '08 term at The University of Texas at San Antonio- San Antonio.

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19_SouthernBlot_Lab19_ - Southern Blot Southern Blot: It is...

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