Unformatted text preview: anesthetization vial with the plug/swab assembly charged with Flynap. • Lightly tap the anesthetizing vial and turn it until the flies no longer cling to the inside surface of the vial. The flies should be anesthetized within about 45 seconds. The flies can now be transferred to a counting tray for observation under the dissecting microscope. CAUTION: Do not over anesthetize the flies. As soon s the flies cease movement immediately remove them from the anesthetizing vial. Flies that have been over anesthetized can typically be recognized since their wings and legs are extended at right angles from their body. Such flies should be considered dead and should not be selected for setting up a new culture....
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- Spring '08
- Genetics, vial, 45 seconds, FlyNap, anesthetization vial