Problem Set 1 - Problem Set 1 (Covers lectures 1-3)...

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Problem Set 1 (Covers lectures 1-3) True/False: Electron microscopy is superior to fluorescence microscopy when studying biological membranes. Explain your answer. False. It depends on what aspect of biological membranes you would like to study. If you want high resolution for imaging a specific structure, electron microscopy will be superior. However, if you want to track the localization of a fluorescently tagged protein to a membrane in real time, fluorescence microscopy will be superior. True/False: Energy (GTP) is not required for an NLS containing protein located in the cytoplasm to enter the nucleus. Explain your answer. False. While GTP is not technically required for the single movement of an NLS containing protein from cytoplasm to nucleus, it is required for the entire cycle of events required to make transport possible. Ran-GTP is required to release proteins from importins within the nucleus and Ran-GTP is essential for the transportation of importins back to the cytosol so that they are available to transport NLS containing proteins. Additionally, GTP or another source of energy is always needed to facilitate active
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This note was uploaded on 05/04/2008 for the course MCB 130 taught by Professor Schekman during the Spring '08 term at University of California, Berkeley.

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Problem Set 1 - Problem Set 1 (Covers lectures 1-3)...

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