CHM 226 - Tech 708 - Column Chromatography

CHM 226 - Tech 708 - Column Chromatography - Tech 708...

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Tech 708 Column Chromatography INTRODUCTION Column chromatography is a technique where a solid is packed into a glass column and a solvent moves through the solid to separate individual compounds in a mixture. This type of chromatography is greatly used for separating slightly larger amounts of compounds in a mixture. In this lab, a mixture of two solids is dissolved and separated using column chromatography. EXPERIMENTAL PROCEDURE A micro-scale chromatography column was prepared by securing it with a clamp and filling it half way with sand. Hexane was added to the column and drained so there was a minimal amount of hexane on top of the sand. A 50:50 mixture of ferrocene and acetylferrocene weighing about 70 mg was evenly mixed and carefully poured into the column, trying to make sure that the mixture was added in one try to ensure consistency. To elute ferrocene, hexane was added to the column, collected, and allowed to elute until the yellow ferrocene band reached the bottom. At this point, a different beaker was used to
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This note was uploaded on 05/04/2008 for the course CHM 226 taught by Professor Jesse during the Spring '07 term at Rhode Island.

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CHM 226 - Tech 708 - Column Chromatography - Tech 708...

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