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Physiology Worksheet - Physiology Worksheet Medium(abbrev...

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Physiology Worksheet Whitney Barter Page 1 of 5 Exercise Enzyme/Substrate/Product Reagents/Indicators/Inhibitors Exer 4-1 MSA TS E = Mannitol dehydrogenase R = None Mannitol Salt Agar S = Mannitol IND = Phenol Red P = Organic Acids INH = 7.5% NaCL Purpose Basis of Selection/Differentation Exer 4-5 EMB TS E = Lactase R = None Eosin Methylene Blue Agar S = Lactose IND = Methylene Blue & Eosin P = Organic Acids INH = Methylene Blue & Eosin Purpose Used for the isolation of fecal coliforms. Basis of Selection/Differentation Exer 4-6 MAC TS E = Lactase R = None MaConkey Agar S = Lactose IND = Neutral Red & Crystal Violet P = Organic Acids INH = Crystal Violet & Bile Salts Purpose Basis of Selection/Differentation Exer 4-7 HEK TS E = Lactase / Sucrase/ Salicinase R = None Hektoen Agar S = Lactose / Sucrose / Salicin IND = Bromthymol Blue / Acid Fuchsin / Ferric Ammonium Sulfate P = Organic Acids INH = Bile Salts. Purpose Basis of Selection/Differentation Medium (abbrev.) Innoc. Method Isolation of pathogenic, catalase + strains of Staphlococcus aureus. This is a selective and differential media. It is selective due to the presence of the inhibitor 7.5% NaCl that inhibits the growth of gram negative cells. It is a differential media due to the presence of the indicator phenol red that turns from reddish pink at pH7 to yellow in the presence of organic acids. This is a selective and differential media due to the presence of the inhibitors/indicators methylene blue and eosin. Both inhibitors inhibit the growth of gram positive cells. Both indicators turn the agar a green sheen in the presence of a heavy producer of organic acid. They produce colonies with a red dot in the center in the presence of small producers of organic acid. Clear/white colonies are produced when the organisms uses the protein in the media. Used to isolate and differentiate members of the Enterobacteriaceae based on the ability to ferment lactose. This is a selective and differential media due to the presence of the indicators/inhibitors. The inhibitors crystal violet & bile salts inhibit the growth of gram positive cells. The indicators neutral red and crystal violet are colorless at pH 7 and become reddish/ pink in the presence of organic acids. Used to isolate and identify Salmonella and Shigella from other gram negative coccobacilli. This is a selective and differential medium. It is a selective medium due to the presence of the inhibitor, bile salts, which inhibit the growth of gram positive cells. It is a differential media due to the presence of the three indicators. The first indicator, bromthymol blue, is a pH indicator that is green at neutral pH. If any organic acids are present then the agar will be turned a yellow color and if the protein in the medium is used then the agar will turn blue-green in color. The second indicator, Acid Fuchsin, is also a pH indicator and is pale pink at neutral pH. If acid is produced the colonies will turn pumpkin orange. The third indicator, ferric ammonium sulfate, combines with dihydrogen sulfide, which is produced during the breakdown of the protein source, to produce iron sulfide which is black in color.
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