unknown project

unknown project - Identification of Bacterial Unknown #82...

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Unformatted text preview: Identification of Bacterial Unknown #82 Staphylococcus epidermidis Allison McGee Submitted: Monday November 19, 2007 Lab Period: Thursday 8-10:50 am Table of Contents Table of Contents.2 Abstract3 Introduction..4 Experimental Procedures and Results..6 Figures and Tables8 Discussion.12 References.14 2 Abstract: The goal of this project was to identify the unknown bacteria assigned to us. Each student was assigned an unknown stock culture of bacteria. The student then had to perform various tests to determine what bacteria was their unknown. Bergeys Manual and Gideon Online were used to help establish known results for the tests, to compare with results achieved by the student. Unknown #82 contained Staphylococcus epidermidis. This was concluded after the performance of five tests. 3 Introduction: The broad goal of this project was to determine what bacteria was in our unknown tube. Many smaller goals came out of this project, such as developing our skills of scientific reasoning and our accuracy in performing the various tests. This project has helped develop our scientific reasoning skills for whatever we may go on to in life. We had to use scientific reasoning to start with our unknown bacteria and determine what tests to perform to narrow the lists of possible organisms all the way down to one. In the scientific and medical fields, they have to use scientific reasoning all the time. Direct applications of this could be determining what bacteria are infecting a patients body or what bacterium could help clean up an oil spill. Scientists and doctors alike must have scientific reasoning skills to use everyday in the laboratory or office, whatever the case may be. To start, the unknown had to be isolated from the mixed culture. Many T-streak plates were performed. Once an organism is isolated, the determination of the organism could begin. The first step in determining what organism was in the unknown tube was to perform a gram stain to determine the morphology of the organism and whether it was gram positive or negative. Using Bergeys Manual, a dichotomous key was developed, Figure 1 that categorized organisms by gram negative or gram positive. Once the organism was categorized, the organism could further be categorized by morphology, as in Figure 2. From there, using Bergeys Manual and Gideon Online, we see what tests distinguish between the most organisms, to narrow it down to one. Unknown tube #82, contains gram positive cocci, this left a very few number of organisms to have to distinguish between. After research, it was determined that the Nitrate test, Catalase test and Voges-Proskauer test distinguish the number of organisms down to the 4 Staphylococcus genus. After this, only the coagulase and mannitol salt tests needed to be done to determine what species of Staphylococcus was in the unknown tube, as seen in Figure 3....
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unknown project - Identification of Bacterial Unknown #82...

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