genChap5a - Chapter 5 (Draft) Gene transfer in bacteria and...

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Chapter 5  (Draft) Gene transfer in bacteria and their viruses. Viruses are not cells. They cannot grow or multiply on their own. Prototrophs can grow on minimal medium?  Composition? Pp208-209. Auxotrophs cannot grow on minimal medium.  Why not? The F plasmid and bacterial conjugation. F plasmid has the loci to allow a bacteria to grow a pilus ( pili, plural.) Pilus is a cytoplasmic extension.   It can contact another bacterial cell and pull the bacteria closer together. DNA can then travel from the pilus into the cell without the F plasmid. It is a one-way transfer. F plasmid may be not integrated into bacterial chromosome. Cell called an F + cell.   In this state a copy of the F plasmid will transfer easily from the F + or male cell  into the F – or female cell. Thus, F + cells can convert many other cells into F+. If the F plasmid is integrated into the bacterial c’some the cell is called an Hfr  cell.   Hfr stands for High frequency of recombination. The integrated F plasmid can break within the integrated region and cause the  movement of a copy of the bacterial c’some in this cell through the pilus into the 
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genChap5a - Chapter 5 (Draft) Gene transfer in bacteria and...

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