Bio - Lecture 14

Bio - Lecture 14 - Sheet1 Page 1 DNA Replication...

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Unformatted text preview: Sheet1 Page 1 DNA Replication Semiconservative-The solved model of DNA showed complementarity-This is also the basis for DNA replication-The duplex can "unzip" and serve as a template for the new strand->This is called semiconservative replication-This form of replication was confirmed by Meselson and Stahl in 1958 by using radioactive nitrogen In Prokaryotes-DNA replication has been studiest most extensively in E. coli-It beings at the origin (OriC) and ends at the terminus-DNA Polymerase (pol) is the enzyme that synthesizes DNA-There are several types (pol I, pol II, pol III, etc)-Every enzyme requires a primer which is a short segment of DNA or RNA with a free 3' group Polymerase-Every pol enzyme adds nucleotide in the 5' to 3' direction->Some pol enzymes are also nucleases-->These are enzymes that cleave DNA-->Endonuclease cuts in the middle-->Exonuclease chews at the ends-The DNA pol enzymes have 3' to 5' exonuclease activity which means they can "proofread" by removing a mispaired nucleot...
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