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Bio - Lecture 14

Bio - Lecture 14 - DNA Replication Semiconservative-The...

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Sheet1 Page 1 DNA Replication Semiconservative -The solved model of DNA showed complementarity -This is also the basis for DNA replication -The duplex can "unzip" and serve as a template for the new strand ->This is called semiconservative replication -This form of replication was confirmed by Meselson and Stahl in 1958 by using radioactive nitrogen In Prokaryotes -DNA replication has been studiest most extensively in E. coli -It beings at the origin (OriC) and ends at the terminus -DNA Polymerase (pol) is the enzyme that synthesizes DNA -There are several types (pol I, pol II, pol III, etc) -Every enzyme requires a primer which is a short segment of DNA or RNA with a free 3' group Polymerase -Every pol enzyme adds nucleotide in the 5' to 3' direction ->Some pol enzymes are also nucleases -->These are enzymes that cleave DNA -->Endonuclease cuts in the middle -->Exonuclease chews at the ends -The DNA pol enzymes have 3' to 5' exonuclease activity which means they can "proofread" by removing a mispaired nucleot
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