BIO 320 Spring 2008 - Discussion 7

BIO 320 Spring 2008 - Discussion 7 - CELL BIOLOGY (BIO 320)...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
CELL BIOLOGY (BIO 320) SPRING 2008 DISCUSSION SHEET 7 _______________________________________________________________________ _ 1. Cell lines A and B both survive in tissue culture containing serum but do not proliferate. Addition of Factor F stimulates proliferation of cell line A but not cell line B. Cell line A produces a normal receptor protein (R), and, in order to see what the role of R might be, you introduce this receptor protein into cell line B, using recombinant DNA techniques. You then test the various cell lines in the presence of serum for their response to factor F, with the results summarized in the following table. Cell line Response A – Factor F Cells do not proliferate A + Factor F Cells proliferate B – Factor F Cells do not proliferate B + Factor F Cells do not proliferate B + receptor R – Factor F Cells proliferate B + receptor R + Factor F Cells proliferate Which of the following can be concluded from your results? Please explain your choices. (a) Binding of Factor F to its receptor is required for proliferation of cell line A but not cell line B. (b) Binding of receptor R to its ligand is required for proliferation of cell line B but not cell line A. (c) Receptor R probably does not bind to Factor F. (d) Cell line B does not normally have a receptor for Factor F. 2. Can signaling through the steroid hormone/steroid hormone receptor pathway lead to amplification of the original signal? If so, how? 1
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
3. Your friend is studying a segment of a newly discovered virus that carries an enhancer of gene expression that confers glucocorticoid (a hormone) responsiveness on genes that are linked to it. He constructs two versions of a reporter gene, one which has only a minimal promoter linked to it (which contains sites for RNA polymerase binding), while the other reporter gene has both a minimal promoter plus the viral enhancer attached to it. The reporter gene allows him to measure the amount of transcription that occurs from each construct. Your friend puts each of these constructs into two different cell lines and examines the activity of the reporter gene in each cell line, as shown in the figure below. He is puzzled by his data and asks for your help in interpreting the findings. A.
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 05/06/2008 for the course BIO 320 taught by Professor Staff during the Spring '08 term at University of Texas at Austin.

Page1 / 7

BIO 320 Spring 2008 - Discussion 7 - CELL BIOLOGY (BIO 320)...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online