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unknown2-1 - Unknown 2 Date Tube 12 Unknown Should Have...

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Unknown # 2 Date: 3/31/08 Tube # 12 Unknown Should Have Been: Proteus vulgaris and Salmonella Grading: Possible Received 1. Description of the test performed 5 _______ (Purpose, equations, what it is) 2. Description of the positive/negative Results for the test (what should we be 5 _______ looking for & interpretation of tests) 3. How your organisms responded to each 5 _______ Test 4. How you deduced what organism you 5 _______ Have Points Possible 20 Points Received _________ Comments:
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Introduction: The four possible unknowns that could have been obtained for unknown number two were: E. coli , Proteus vulgaris, Salmonella, and Shigella. For this unknown, everyone had either Salmonella or Shigella and Proteus vulgaris or E.coli so that everyone had a mixture of two unknowns. The principle I used to differentiate between the various organisms was the H2S produced by the Salmonella on the HE plate as well as the fact that it tests positive for urease. For Proteus vulgaris , I differentiated it from the other organisms because it only ferments lactose and E. coli does not. This made it quite obvious that my unknowns were Proteus vulgaris and Salmonella because P. vulgaris fermented lactose and Salmonella produced H2S on the HE plate. I was successful in finding Proteus vulgaris and Salmonella as my two unknowns because of the MC plate and the HE plate. Results: From left to right: MacConkey’s (MC) Agar, Hektoen Enteric (HE) Agar, Xylose Lysine Desoxycholate (XLD) Agar, Russel Double Sugar (RDS) Slant. On the MC plate, whitish-clear colonies appeared as well as pinkish- red colonies. On the HE plate, green colonies with black centers appeared. On the XLD plate, pinkish-red colonies with black centers appeared. On the RDS slant, there was pink on the top and yellow on the bottom.
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