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unknown4final1 - Unknown 4 Date Tube 7 Unknown Should Have...

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Unknown # 4 Date: 4/14/08 Tube # 7 Unknown Should Have Been: Staphylococcus saprophyticus Grading: Possible Received 1. Description of the test performed 5 _______ (Purpose, equations, what it is) 2. Description of the positive/negative Results for the test (what should we be 5 _______ looking for & interpretation of tests) 3. How your organisms responded to each 5 _______ Test 4. How you deduced what organism you 5 _______ Have Points Possible 20 Points Received _________ Comments:
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Introduction: The three possible unknowns that could have been obtained for unknown number four were: Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus saprophyticus. The principle I used to differentiate between the various organisms was the fermentation of mannitol on the MSA plate as well as the resistance to Novobiocin. This made it quite obvious that my unknown was S. saprophyticus because that is the only one that is supposed to test positive for Novobiocin resistance. I was successful in finding my unknown to be S. saprophyticus because the results from my streak plates were very definitive. Results: First row from left to right: SM110 plate, MSA plate, and Blood agar plate. Second row from left to right: DNase plate and Coagulase test. The SM110 plate had white bubble-like colonies. The MSA plate turned from red to yellow with white bubble-like colonies. The blood agar plate had no susceptibility to Novobiocin and no types of hemolysis. The DNase plate had growth all over and was not green due to suspected Proteus vulgaris contamination. The coagulase test was negative as there was no lump, just a straight slant when turned to the side. Discussion: SM110 plate: The purpose of the SM110, or Staphylococcus Medium 110, plate was to test for possible color changes as well as to culture amounts of the unknown Staphylococcus for future tests and to test for salt tolerance. This plate contained mannitol and 7.5 percent sodium chloride. There were no real positive or negative results, simply growth as well as a possible color change to yellow or orange. The SM110 favors pigmentation of S. aureus . Pictured above is this plate. The left colony was unknown number seven and the right unknown was unknown number two. Unknown number 7 was white and Unknown number 2 was yellowish-orange. This plate
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