Lecture_3A_-_DNA_synthesis_S2008

Lecture_3A_-_DNA_synthesis_S2008 - Sliding DNA clamp of DNA...

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DNA replication
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DNA replication:Meselson and Stahl
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DNA replication:Meselson and Stahl
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Questions about replication
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Theta form of DNA
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Bidirectional replication begins at a unique place 1 2 3 4 5 6 7 8 9 10
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Origin of replication
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Initiation of replication
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DNA replication proteins of E. coli required at origin of replication
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DNA replication is primed by a short stretch of RNA
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DNA synthesis
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DNA synthesis – mechanism
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Base pair geometry in replication
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3' 5' exonuclease activity
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E. coli DNA polymerases
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E. coli DNA polymerase III
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Unformatted text preview: Sliding DNA clamp of DNA polymerase III Apparent DNA replication Actual DNA replication Synthesis of Okasaki fragments Joining Okasaki fragments Nick translation Ligation DNA replication proteins of E. coli required at replication fork A dimeric DNA polymerase III A dimeric DNA polymerase III Position of termination sequences Creation of catenanes Chromosomal partitioning Polymerase chain reaction (PCR) PCR PCR to clone DNA...
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Lecture_3A_-_DNA_synthesis_S2008 - Sliding DNA clamp of DNA...

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