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SBP 3403 BASIC MEDICAL MICROBIOLOGY PRACTICAL MANUAL CELL BIOLOGY UNIT DEPARTMENT OF BIOMEDICAL AND HEALTH SCIENCES FACULTY OF MEDICINE AND HEALTH SCIENCES UNIVERSITI PUTRA MALAYSIA
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SAFETY RULES IN MICROBIOLOGY LABORATORY Maintaining a clean laboratory setting is crucial to prevent contamination of experimental procedures by microorganisms from exogenous sources. This is because most microbiological laboratory procedures require the use of living organisms that is capable of producing disease. Thus, microbiology students are required to develop aseptic techniques in the preparation of pure cultures and investigation of a clinical specimen. The following basic steps should be observed at all times to minimize the contamination by microbial flora of the laboratory environment and to prevent accidental injuries: 1. Upon entering the laboratory, place books, beg and other paraphernalia in the specified location. 2. Wash your hands with liquid detergent and rinse with 95% ethyl alcohol, and dry them with paper towels upon entering and before leaving the laboratory. 3. Wear a laboratory coat, gloves and closed shoes at all times in the laboratory setting. 4. Tie back long hair to minimize its exposure to open flames. 5. Wipe bench tops with 95% ethyl alcohol at the beginning and after finishing each laboratory session. 6. Do not place contaminated instruments, such as inoculating loops, needles and pipettes on the bench tops. Loops and needle should be sterilized by incineration and pipettes should be disposed in the disposable bin. 7. Never apply cosmetics or insert contact lenses in the laboratory. 8. Smoke, eat and drink are prohibited in the laboratory. 9. Carry cultures in a test tube rack and never remove media, equipment and bacteria cultures from the laboratory. 10. Report accidental cuts or burns to the instructor immediately. 11. Pipetting must be carried out with the aid of mechanical pipetting device only. 12. Speak quietly and avoid unnecessary movement around the laboratory to prevent distraction that may cause accidents. 13. On completion of the laboratory session, place all cultures and materials in the disposable area as designated by the instructor.