2nd Midterm2015 (7) - 2nd Exam Biology 3310 Cell Phys 2015...

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2 nd Exam Biology 3310, Cell Phys. 2015 Version A Name _______________ The exam has 102.5 total points; multiple choice questions are worth 1.75 pts each (75.25 pts total), short answer questions are worth 27 pts. 1. RNA polymerases I, II and III respectively transcribe: a. mRNA, tRNA, rRNA b. mRNA, rRNA, tRNA c. rRNA, tRNA, mRNA d. rRNA, mRNA, tRNA 2. The distinct characteristics of different cell types in a multicellular organism are produced mainly by the differential regulation in the: 3. Transcription in bacteria differs from transcription in a eucaryotic cell because 4. What is a riboswitch? Questions 5 and 6 deal with the prokaryotic promoter sequence shown below 5’-ATCGGTACCG TTGACA ATCGACTAGGCTAGCT TATATT GTACGTAGGTC-3’ 3’-TAGCCATGGC AACTGT TAGCTGATCCGATCGA ATATAA CATGCATCCAG-5’ 5. The bold sequences (TTGACA and TATATT) represent a. TATA boxes recognized by RNAP II b. -35 and -10 boxes recognized by the sigma factor of RNAP c. Binding sites for general transcription factors d. Binding sites for the small subunit of the ribosome 6. If transcription starts at the arrow, the sequence of the transcribed RNA will be: 7. You have identified a protein factor that when added to in vitro protein synthesis reaction increases the incorporation of 35 S-methionine from 4 pmoles/minute to 8 pmoles/min. When you centrifuge these reactions and measure polyribosomes and free ribosomes, you observe that more of the ribosomes are present as polyribosomes for the reactions that contain your stimulatory factor. Which explanation is consistent with this result?
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