Unit 5 Chromatography - Unit 5 Chromatography Tuesday 9:13...

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Unit 5 Chromatography We talked about how to develop the method for that data - Looked at how we will select a sample - How to develop quantitative analysis (make calibration curves) - Had a general idea to detect/ analyse sample - Calibration curves (standard addition and external calibration) Quant. How much was it Quantitative (how much is it) and qualitative (what is it) analysis - Unit 1 Started to look at the different types of reactions that we can explore in terms of being able to dissolve an acid and measure something - They have to be distinguished, there are different types of rxns and they are used for different types of purposes The different types of titrations - If substances have acidic or basic properties Can use visual detection And pH indicators Equilibria of acid and base - Two organic compounds cannot bind to each other When there is the binding of the metal there is a colour change which is considered the indication Detection of metals (metal indicators or also known as ligands. They are called metal indicators because they bind to a metal, 2A phenol-orange) When using EDTA Complexometric rxns and titrations (metals which induces a change in colour) - Precscence of halides (silver nitrate) or the silver Fluorosein - complexes with silver and turns pink Missing There are two different types of methods that are visually detected here: Two units talking about this unit 2 and 4 Electrical (quantitative) Precipitation (had to do with experiment 3) - Potentiometric (something we're measuring not really a tirtration it deals with generating a signal Unit 3 AFTER YOU HAVE DIFFERENT TYPES OF RXNS THAT ARE TAILORED TO DETECT THE ANALYTES THAT WE ARE INTERESTED IN What we need is the generation signal to collect the quantitative or qualitative data: Visual type of detection (fast and cheap and efficient) but it may not be that quantitative the unit 3 things are all visual due to colours change) a. They could be measured as charge or the voltage and then you convert it so it actually relates to the signal Electrical type of detection for example pH or temperature What types of signals could you absorb? - Signal UV - V1 absorption Fluorescence IR spectroscopy Unit 2 spectroscopy - interaction of a compound with the radiation. Primary focus is the: UV - V1 absorption. Way to generate signal - Use of electrodes: able for example ion selactive electrode (glass) Measure potential that provides the information the first kind (which is not used a lot because there are a lot of side reactions that occur) second kind (metal solid wire) Important to know: the analyte does not undergo a redox reaction which makes it widely used for a variety of ions since not everything can undergo redox reaction ion selective electrode also known as the indicator electrode (most important ex. Glass electrode) The one that will respond to the analyte concentration is the indicator electrode: Unit 4 Potentiometry -
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