imm340 julius tutorial - How do we make a congenic strain...

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How do we make a congenic strain? o The idea of backcross and crossing over o Dilute dna in the strain that you are using – the black 6 in this case o Balbc except for igb o Know figure I-2: o 1:1 distribution of pups o know the math behind the backcross o regardless of backcross o the amount of dna o 0.1% is the gene that you are selectin g o the distribution of pups what expression in terms of allotype in terms of backcross- 50% of heterozygous, 50% homozygous of the background strain , 100% expressing ig A What is congenic strain? Need to understand the notation for Figure I-8 Mhc+ antigen tell tcr specificity Need to understand julius’s lectures well Pan experiment: o Need to understand the concept of haplotype and mch class one is for K and D o Have a petri dish that you cover with what will be the target’s cells in the pan experiment o Isolating means to enrich the t cell population with which you are interested in o Apc is in general haplotype d o
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