3221_MidtermReview(1) (1) - Lab 1 Hand washing Learning...

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Lab 1: Hand washing Learning Objectives: Understand the importance of hand washing Note differences in soaps and alcohol based sanitizers and how they are effective at removing bacteria Learn basic terms regarding bacteria as well as important figures that contributed to Microbiology
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Ignaz Semmelweis Introduced handwashing in chlorinated lime solutions at Vienna General in 1846 Documented decrease in incidence of puerperal fever upon implementation of handwashing protocol Ridiculed at the time; subsequently fired! in 1865 he was committed to an asylum Ironically died of septicemia after receiving a beating from the asylum guards
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Joseph Lister Father of antisepsis Cleaned hands and instruments in a carbolic acid solutioin Principle is the cornerstone of modern surgery Showed sterilized instruments could be left in patient Invented “stiches” Lister got his idea of sterilization from Pasteur’s work on pasteurization as a means of lowering the microbial load in dairy products Concluded that general heating would not work well with his patients , went the chemical disinfectant route.
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Lab 1: Epidemiology Learning Objectives: Figure out what epidemiology is and how it is studied Learn terms associated with Epidemiology Be able to describe methods associated with transmission of infections Understand how easy it is to actually transmit microorganisms from person to person
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Lab 2 Learning Objectives (Streaking for Isolation/Microbes in the Environment Realize how abundant microbes are in our environment Note important contributors to Microbiology, especially those attributed to microscopy, the Germ Theory of Disease, and aseptic technique Learn about aseptic technique and how to apply it especially in medical settings Learn and understand methods for isolating and enumerating bacteria Become familiar with reading and using pipets Successfully perform cfu/mL counts
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Germ Theory of disease The theory that proposes that microorganisms are the cause of many diseases. Louis Pasteur Disproved theory of spontaneous generation Robert Koch Developed postulates that validated the germ theory of disease
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Koch’s postulates
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What can we tell from a plate? Whether or not we have a pure culture Colonies of a single morphological type are all that are present upon our streak plate If you have several different morphologies (i.e. different sizes, shapes, colors, etc.., then you have a mixed population ) Could you eventually get a pure culture from a mixed population isolation streak? How? Nutrient agar plate inoculated with a swab from a door handle *Note the different morphologies (i.e.
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