ch 21 micro - Ch.21 SHORT ANSWER 1 Explain at least three...

Info icon This preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Ch.21 SHORT ANSWER 1. Explain at least three ways in which metagenomes may miss organisms in the environment. Some organisms can be apart of the rare biosphere, which is a species of such low abundance in the soil that the metagenomic screen does not pick them up Failure of the filters and DNA isolation. Organisms may unintentionally be lost by filtering procedures Failure of the PCR primers. SSU rRNA miss many species whose sequence is beyond range of primer sequence 2. You make two different kinds of metagenomes—one to measure diversity from only 16S rRNA DNA sequences, and one from the entire DNA that does not require amplification. You observe 1,000 times more 16S rRNA gene sequences from the first metagenome than the second. However, you find that the number of species according to the 16S rRNA gene sequences is 10 times more in the second metagenome than the first. Why? 3. Describe the steps in sequencing a metagenome. Sample microbial target community Filter sample Break open cell and isolate DNA Clone DNA fragment Read DNA sequence Build scaffold and build genomes 4. Explain the difference between assimilation and dissimilation. Assimilation o Organisms acquire an element to build into cells Dissimilation o Breaks down organic nutrients into inorganic minerals through oxidation 5. What two factors most differentiate aquatic and terrestrial ecosystems? Why?
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Aquatic most CO2 fixation and biomass production performed by phototrophic bacteria main consumers are protists and viruses, they lyse most marine cells before any
Image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern