Lecture 10 Cell Migration and Adhesion October 26 2016

Lecture 10 Cell Migration and Adhesion October 26 2016 -...

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1 Lec 9 Oct 19-24 Perlecan and Agrin Small leucine-rich proteoglycans (SLRPs): Decorin and collagen Fibrillogenesis Biglycan and osteogenesis Lec 10 Oct 26 Cell adhesion and migration
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2 Figure 16-1 Molecular Biology of the Cell (© Garland Science 2008) Cell adhesion and migration: focus on actin dynamics Microtubule Actin filaments DNA (DAPI) Focal Adhesion sites: involved in cytoskeletal dynamics & signal transduction cascades
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3 “Seven protein complex called ARP2/3 (light and dark blue) attaches to the side of an existing actin filament (orange) forming the base of a branch filament. Two proteins in the ARP2/3 complex (dark blue) are similar to actin monomers and are positioned as the first two monomers of the branch.” 13305&menuType=1&action Actin: structure, function and dynamics + end - end
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4 Co-discover of actin Noble Prize winning physiologist Albert van Szent-Gyögyi Nagyrápolt- With Brunó Ferenc Straub Institute of Medical Chemistry, University of Szeged, Hungary 1931 Actin was first observed experimentally in 1887 by W.D. Halliburton Nagyrápolt
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5 A moving cell exerts traction forces on the substratum A keratinocyte plated on a thin silicon membrane exerts forces from contraction of the cell body, causing the membrane to buckle Silicon membrane Leading edge of the keratinocyte Lateral contractile forces
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6 Schematic of the actin cytoskeleton in a migrating melanoma cell Small and Resch (2005) Curr Opinion Cell Biol 17:517, Fig. 3 Severing (scissors) Gelsolin and cofilin Myosin II Actin contractile assemblies generating lateral forces Arc-like arrays (ar) Lamellipodium (lp): pushing force Contractile peripheral bundles Focal adhesion site Microspike bundle Direction of migration
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7 Mode of cellular protrusion as determined by ECM dimensionality 2D 1D 3D 3D 3D Current Opinion Cell Biol (2013) 25:642-649 Modified Figure 2 Classic hand mirror morphology: activated β 1 integrin adhesions, phalloidin (actin) Fibroblast cells eGFP N-VASP (actin nucleator) 1D micropatterned line) Aligned 3D-Cell derived matrix (CDM) Phalloidin Fibronectin Paxillin lamellipodia in non-linear 3D collagen. Lobopodia (LO) and lateral blebs shown by eGFP-actin (a) (b) (c) (d) (e)
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8 Mode of cellular protrusion as determined by ECM dimensionality. (a) NIH/3T3 fibroblast demonstrating a classic hand-mirror morphology on a 2D substrate; red is phalloidin staining and green shows activated β 1 integrin adhesions. (b) eGFP-VASP (left) and phase contrast (right) image of a NIH/ 3T3 fibroblast migrating along a 1D micropatterned line. (c) NIH/3T3 fibroblast within a 3D-CDM showing staining for F-actin (phalloidin, cyan), paxillin (yellow), and fibronectin (magenta). (d) eGFP-actin expressed in a human foreskin fibroblast illustrating lamellipodia in non-linear 3D collagen. (e) Lobopodia (LO) and lateral blebs (B) shown by eGFP-actin as a human foreskin fibroblast migrates through a linear-elastic 3D-CDM. Scale bars: 10 mm.
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  • Fall '09
  • RINGUETTE
  • cell biology, FN, focal adhesion, Vinculin

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