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Harada, activation of 5ht in anxiety in rats

Harada, activation of 5ht in anxiety in rats - Some of the...

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Harada, K., Yamaji, T., & Matsuoka, N. (2008, March). Activation of the serotonin 5-HT receptor is involved in the enhanced anxiety in rats after single-prolonged stress. Pharmacology, & Behavior , 89 (1), 11-16. Activation of the serotonin 5-HT 2C receptor is involved in the enhanced anxiety in rats after single-prolonged stress. This study used a single-prolonged stress (SPS) on rats to examine behavioral changes that develop over time. The purpose was to use rats as a model of post-traumatic stress disorder (PTSD) in humans. Eight-week-old male rats were exposed to a 2 hour restraint, a 20 minute forced swim and ether anesthesia(SPS event). One or seven days following the SPS event, the rats were decapitated and the brain was removed for sectioning. Researchers applied this same procedure to the control rats. Tissue from the hippocampus, amygdala and anterior cingulate cortex were removed for RNA isolation. Serotonin (5-HT) concentrations in these brain areas were also calculated.
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Unformatted text preview: Some of the rats “were subcutaneously administered FR260010 seven days after the SPS. Thirty minutes after this administration, they were subjected to five inescapable scrambled electric footshocks in a chamber… Twenty-four hours after the conditioning session, the animals were again placed in the same chamber, this time without footshock”(308). The behaviors of individual rats were recorded and examined based on whether the rat froze or moved every 10 seconds. The tissues from the hippocampus, amygdala, and anterior cingulate cortex were compared between the SPS and control rats. Of these three tissues the amygdala had the greatest number of differentially expressed genes. The 5-HT receptor mRNA also had a significant increase in the amygdala. “Comparative microarray analysis using RNA from the brains of both SPS rats and unstressed controls showed that the expression time course of 31 genes corresponded to the behavioral changes of SPS rats…...
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