prelab 5 - the compound travels, given the distance...

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John Goodson Analysis of Analgesics by Thin Layer Chromatography Introduction: In this experiment, the active ingredients of several commercial analgesics will be analyzed in order to determine their active ingredients. Analysis will be carried out through thin layer chromatography, a solid-liquid form of chromatography. TLC relies on the equilibrium partitioning of a compound between a stationary phase and a mobile liquid phase. The compounds to be analyzed are adsorbed onto a plate, usually by using microcapillary spotters. A polar solvent is allowed to move up the plate by capillary action. The polarity of the solvent causes interactions with the compounds, interfering with the solid phase and moving them into the mobile phase, drawing them up the plate with the solvent. Different compounds have different polarities and will move into the mobile phase at different rates, allowing analysis of the different compounds. The retention factor, or R f value, is a constant of a compound, determined by the distance
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Unformatted text preview: the compound travels, given the distance traveled by the solvent. R f = Distance Traveled by the Compound Distance Traveled by the Solvent In this experiment, UV light will be used to visualize the spots. The UV light will cause the plate to glow and the compounds absorbed will appear as dark spots, which can be outlined to give a permanent indication of the compound locations. John Goodson Table of Reagents: Safety: Spotting capillaries are made of very fine glass and should be handled with care and disposed of properly. Do not look directly into the UV lamp or point it at other people, as it can cause serious eye damage. Also try to avoid excessive exposure of the hands to the UV light. Compound Amount Properties Acetylsalicylic Acid compound Acetaminophen compound Ibuprofen compound Caffeine compound Dichloromethane 2mL compound solvent Ethanol 2mL compound solvent Ethyl Acetate (.5% Acetic Acid) Enough to cover bottom of chamber eluting solvent...
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prelab 5 - the compound travels, given the distance...

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